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purified pp5  (Millipore)


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    Structured Review

    Millipore purified pp5
    Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) <t>PP5</t> were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .
    Purified Pp5, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purified+pp5/purified+pp5/pmc05128750-165-40-41
    Average 90 stars, based on 1 article reviews
    purified pp5 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins"

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins

    Journal: Marine Drugs

    doi: 10.3390/md14110207

    Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) PP5 were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .
    Figure Legend Snippet: Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) PP5 were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .

    Techniques Used: Activity Assay, Control

    Calculated IC 50 values and relative potencies for natural OA, DTX1, and DTX2, and synthetic DTX2, and 2 -epi -DTX2, based on PP2a, PP1, and PP5 inhibition.
    Figure Legend Snippet: Calculated IC 50 values and relative potencies for natural OA, DTX1, and DTX2, and synthetic DTX2, and 2 -epi -DTX2, based on PP2a, PP1, and PP5 inhibition.

    Techniques Used: Inhibition

    Related Articles

    Activity Assay:

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins
    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Purification:

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins
    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Recombinant:

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins
    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Control:

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins
    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Inhibition:

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins
    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).



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    Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) <t>PP5</t> were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .
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    Image Search Results


    Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) PP5 were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .

    Journal: Marine Drugs

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins

    doi: 10.3390/md14110207

    Figure Lengend Snippet: Effect of various DSP toxins on protein phosphatase activity. The activities of protein phosphatases ( A ) PP2a, ( B ) PP1, and ( C ) PP5 were assessed in the presence of various concentrations of natural OA, natural DTX1, natural DTX2, synthetic DTX2, or synthetic 2- epi -DTX2. All data (mean ± SE; n = 3 or 4) were normalized to the control. Three-parameter, variable slope, non-linear dose-response analysis was performed and IC 50 and 95% confidence interval values were calculated . Note: Some of these data have been published previously in Pang et al. .

    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Techniques: Activity Assay, Control

    Calculated IC 50 values and relative potencies for natural OA, DTX1, and DTX2, and synthetic DTX2, and 2 -epi -DTX2, based on PP2a, PP1, and PP5 inhibition.

    Journal: Marine Drugs

    Article Title: Structure–Activity Relationship Studies Using Natural and Synthetic Okadaic Acid/Dinophysistoxin Toxins

    doi: 10.3390/md14110207

    Figure Lengend Snippet: Calculated IC 50 values and relative potencies for natural OA, DTX1, and DTX2, and synthetic DTX2, and 2 -epi -DTX2, based on PP2a, PP1, and PP5 inhibition.

    Article Snippet: Briefly, the assay tests the ability of toxins to inhibit the activity of purified PP2A (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-111; human red blood cells), purified PP1 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-110; rabbit skeletal muscle), or purified PP5 (EMD Millipore Corporation, Darmstadt, Germany, cat. #14-778; recombinant enzyme expressed in E. coli cells) against the fluorimetric substrate, 6,8-difluro-4-methyl umbelliferyl phosphate (DiFMUP; 50 μM final) (Invitrogen ThermoFisher Scientific, Waltham, MA, USA, cat. #D6567).

    Techniques: Inhibition

    TABLE 2

    Journal: The Journal of Biological Chemistry

    Article Title: The Molecular Chaperone Hsp70 Activates Protein Phosphatase 5 (PP5) by Binding the Tetratricopeptide Repeat (TPR) Domain *

    doi: 10.1074/jbc.M113.519421

    Figure Lengend Snippet: TABLE 2

    Article Snippet: The two spectra have the same intrinsic signal to noise ratio ((254/169) 2 = 2.25). p-Nitrophenyl Phosphate Assay Purified PP5, Hsp70, and Hsp90 were immobilized in 4HBX 96-well plates (Thermo Scientific) and diluted with ELISA buffer (BioLegend).

    Techniques: